FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtSPL11_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtSPL11_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786220
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68360.18054946622224805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG8800.014.71554670
TACGTGT23300.011.56623870
CAGTACG28500.011.42092169
GTACGTG23050.010.62894469
AGTACGT28600.010.27946670
TCAGTAC36250.09.84816369
GTCAGTA46100.09.18632470
GTACGGG3854.285133E-49.09104467
AGAGCGG17300.08.90151570
GCGTCGG13150.08.78305270
TCGTGTT65650.08.21000370
TGTCAGT55000.08.20899669
GTGTGTG36300.08.00255770
GTAGGGG18050.07.949941670
GTGTCAG64450.07.928430670
ACGTGTA23200.07.693876769
GTGTGTC76050.07.68564469
TGTGTCA80300.07.27877470
AGTGTGT118100.07.08289969
AGAGTGT142250.06.79078369
Produced by FastQC (version 0.11.5)