FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtLHY_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtLHY_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4038055
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57710.14291533919176436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG11250.09.95549870
TCGTGTT57350.09.82557570
ATCGCGT3900.0048456738.0791771
GTTAAGA92550.07.828155570
GTGTTAA109000.07.770500769
TGTTAAG88850.07.681345569
AGTGTTA117100.07.20311468
GATTAGT40850.07.196931469
GGGAAAA44500.07.07861170
GAGTGTT126450.06.69826170
CTCTGGG5300.0099044826.603408318
CGTCGTT11301.0570511E-76.50438770
AGATTAG46500.06.473000568
AGAGCGG10501.3600566E-66.33329770
AAGAGTG162100.06.304713270
TAAGATT85900.06.19321270
AGATTAT49300.06.176434570
AAGATTA76600.06.168295469
AGAGTGT138900.06.148272567
AGTGTAG20350.06.01962170
Produced by FastQC (version 0.11.5)