FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtLHY_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtLHY_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087278
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61640.19965808067818966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG6750.013.48056570
CCGGGTG4002.2646418E-913.12410670
CCTGGTG16700.012.36442970
GTCCGGG6057.4578566E-1110.99098869
GTAGGGG12300.09.95867270
CGTCCGG5805.178117E-89.65451668
CGGGTGT5109.091673E-79.6071969
TCCTGGG5502.4755536E-79.54480670
TCGTGTT50100.09.50035270
CTGGTGT20000.09.44935770
ACGTCCT25000.09.23937168
GTACGGG3050.0059912079.179852566
TACGTCC36250.09.1717970
GTGTACC8851.0913936E-119.09542770
GTCCTGG32600.08.91043770
TGTACGG15350.08.89190470
GCGTCGG7909.931682E-108.86015770
GTACGTC39250.08.2924368
CGTCCTG32650.07.932079369
ACGTGCG4000.00610140157.87446470
Produced by FastQC (version 0.11.5)