FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtERF1_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtERF1_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478000
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86140.19236266190263512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGT49900.08.97815370
AGATTAG55900.08.45268569
GCGTCGG12650.08.30057870
GTAGGGG16950.08.259768570
AGATTAT40750.08.2455870
TCGTGTT72300.08.1813770
GGGAAAA50250.08.0797870
AGAGCGG16550.08.0364370
GTGTTAA132600.07.65476970
TAAGATT91050.07.265398570
ATTAGTG44100.07.222300569
GTTAAGA110500.07.19012369
TGTTAAG107400.07.16953868
AGTGTTA145600.07.01930769
AGTGTAG26400.06.894014469
AGCGTCC7158.367162E-56.853297770
GAGTGTT160500.06.78208170
AAGATTA81600.06.777034369
TGTTAAT44150.06.7385470
TAGTGTA39800.06.595548669
Produced by FastQC (version 0.11.5)