FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtERF1_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtERF1_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875307
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86010.22194370665343416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG10300.016.65010670
TACGTGT20750.012.14430370
GTACGTG23000.011.41293169
GTGTCAG66850.010.62803170
CAGTACG28750.010.59120169
GTAGGGG26800.010.3169370
AGTACGT27800.010.1976170
GCGTCGG17800.09.83123870
GTACGGG5603.2139542E-79.37490869
TGTGTCA76350.08.9848569
TCAGTAC34950.08.71236169
GTGTGTC76500.08.60122468
GAGCGTG9152.055458E-108.4151170
TGTCAGT53300.08.40506370
GTCAGTA43800.08.39032968
ACGTGTA23400.07.777601270
AGAGCGG31050.07.777601270
GTGTGTG46350.07.626579370
GGGTGTC11202.0736479E-107.499926668
AGTGTGT122000.06.91386767
Produced by FastQC (version 0.11.5)