FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtCAMTA1_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtCAMTA1_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924200
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81810.2084756128637684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG11050.019.95446470
TGTACGG19300.014.68890870
CCGGGTG5150.013.59203970
GTCCGGG7700.011.36347268
CGTCCGG7800.010.76907567
ACGTCCT40500.010.629477569
CCTGGTG28000.010.49984870
TACGTCC61500.010.18684670
TCCGGGT7705.456968E-129.99985669
GTACGTG14600.09.82862670
CTGGTGT31150.09.7751470
GGGAAAA44050.09.69339170
GCGCGGG2900.0039521119.6552834
GTACGTC67450.09.18444969
CGTCCTG52950.08.65898870
GTACGGG4905.7138885E-58.57130569
TCGTGTT68200.08.57025870
GTCCTGG47700.08.43803470
TGTACGT94950.08.40430369
AGTGTAA38500.07.99988570
Produced by FastQC (version 0.11.5)