FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtCAMTA1_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtCAMTA1_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755640
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65500.17440436250545846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG7850.012.92917470
GTAGGGG16000.010.49938170
TACGTGT25900.010.26966570
GTACGTG27650.09.61968670
CAGTACG32100.09.37701869
GTACGGG4502.0995349E-59.33278370
TGTCAGT57950.08.99860770
AGTACGT31300.08.9451670
TCGTGTT63600.08.80451370
GTCAGTA49800.08.43379969
GTGTCAG68200.08.10856569
TCAGTAC41200.07.73012770
GTGTAGA27800.07.679403370
GGCAGTA5652.9864634E-47.43368568
AGAGTGT141750.07.432155669
ACGTGTA26500.07.396282768
GAGTGTG120800.07.387809870
TCAGTAA20200.07.276798270
GTGTGTC79800.07.14917767
GGGAAAA37800.07.036622570
Produced by FastQC (version 0.11.5)