FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtARF2_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtARF2_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948220
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86990.2203271347594612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG16800.011.04148370
GTAGGGG26250.010.66648970
GATTAGT42350.08.76018470
AGATTAG52050.08.13637569
TCGTGTT87500.07.799870570
TAAGATT76800.07.747266370
GTTAAGA88750.07.650674369
GAGCGTG10756.91216E-107.48824870
GTGTTAA98100.07.278166370
TGTTAAG85900.07.252590769
AGAGCGG26900.07.025905670
GAGTGTT134350.06.98173669
AAGATTA64300.06.858448569
ATTAGTG38250.06.771129670
TAGTGTA32150.06.6407269
AGATTAT30900.06.456203570
AGAGTGT148250.06.445168568
AGTGTTA108700.06.40751869
AGTGTAG24050.06.403219770
CGCTCAG6050.0049712736.36385327
Produced by FastQC (version 0.11.5)