FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtARF2_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtARF2_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612862
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77570.21470512851030568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG9550.014.65892170
TGTACGG16350.013.69959170
CCGGGTG4409.3496055E-911.93135969
CGGGTGT6209.094947E-1211.28973370
GTCCGGG6755.682523E-99.33297569
CCTGGTG21800.09.31156969
GTAGGGG16600.09.27662470
GTACGTG14400.09.23562970
CTGGTGT26100.09.11829870
TACGTCC44150.08.87835970
ACGTCCT31650.08.51468269
ACGTGCG5007.239667E-58.39956170
CGTGCTG9001.3224053E-98.16635369
AGTGTAA29050.07.95139270
GTACGTC49850.07.933387370
TGTACGT72100.07.766692669
GCGTCGG10951.2732926E-107.67083270
TACGTGC11605.638867E-117.542814369
GTGTACG87200.07.50544669
TCGTGTT61700.07.430730370
Produced by FastQC (version 0.11.5)