FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14524327
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG679110.467567275234164No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291070.20040171224456732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG125700.019.598411
AGGGGGG50700.018.9384021
TATGCCG43000.014.573668548
ATGCCGT39000.014.09414749
TGCCGTC45550.011.83705250
CGTATGC54300.011.35070846
GTATGCC56350.011.30694547
GCCGTCT36650.011.27263151
CTCGTAT51450.010.20519444
TCGTATG69850.09.021337545
TTGGGGG48900.07.9464474
TCTCGTA68000.07.413110343
CCGTCTT61250.07.202647752
CCAGTCC57350.07.13932470
ATGCCGG20000.06.652078649
GAGCACC85800.06.44428170
ATCTCGT83000.06.41071342
TCCAGTA57950.06.280360770
TAGGGGG22150.06.005774
CAGTCAC670750.05.79117870
Produced by FastQC (version 0.11.5)