FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_pIXHALO_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_pIXHALO_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538336
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5150.013.59208548
TGCCGGG3052.9382554E-612.62281350
TGCGGGG2801.6957953E-512.49986451
ATGCCGG4003.184323E-812.249866549
GTATGCC6401.8189894E-1110.9373847
TGCCGTC5451.6989361E-910.91731350
CGGGGTG3257.7709316E-510.76911353
CTCGTAT8750.010.3997444
CGTATGC5057.1193426E-810.39607346
ATCGGGG4158.032615E-610.12022942
ATGCCGT5653.4508048E-89.91139649
TGCCGGC3300.00106675439.54535150
GGGGGTG5653.6570782E-79.29167259
GCCGGTC3400.00140145189.26447440
AATCGGG7858.8039087E-108.91697441
CAGAAAT64850.08.58100637
GAGCACC13550.08.52365170
AATCGCG5754.621632E-68.52152541
GGGTGTA5351.7226346E-58.5044661
AAGAGCC21250.08.39967170
Produced by FastQC (version 0.11.5)