FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_pIXHALO_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_pIXHALO_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4016353
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT47660.11866486835196009TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT16350.034.03639249
TATGCCG16550.033.62507248
GTATGCC17850.032.3526547
TGCCGTC16000.031.93721250
GCCGTCT13900.031.47453751
CGTATGC19150.030.52229946
TCGTATG22500.024.732845
CTCGTAT20050.022.34365844
CCGTCTT20350.021.84255452
TCTCGTA24700.016.72028743
CTGCTTG19600.015.53518659
GATCTCG28850.015.40694841
ATCTCGT30700.014.02249942
CGATCTC32150.013.934381540
CTTCTGC22900.013.75533356
CGTCTTC28550.013.48499153
GCGTGAA6950.012.58981461
TGCTTGA26800.012.145411560
GCTTGAA28550.011.7687261
TGCCGGC3903.0270712E-711.6665650
Produced by FastQC (version 0.11.5)