FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmTCP11_retic_NYU6-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmTCP11_retic_NYU6-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12984453
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG41550.022.40662242
CATCGGG65250.020.11468141
GCATCGG78700.017.29964840
CGCATCG114550.015.58215339
GCGCATC137850.015.43651338
TGCGCAT177800.013.38534437
TCGGGGT31900.011.63003343
CGGGGGT28950.011.36436244
ATGCGCA236950.011.1222436
GCGGGGT27950.011.01966252
CGGGGTT30500.010.09830744
ATCGCGG25900.09.99994842
CGGGGTG26700.09.96253353
TCGGGGG40000.09.88744743
AATGCGC288850.09.72961270
GCATCGC43250.09.38716140
TAATGCG335000.09.266829570
CATCGCG39150.09.02925741
GCGCATG38600.08.70434970
CGCATCT48200.08.495543539
Produced by FastQC (version 0.11.5)