FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmTB1_retic_NYU4-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmTB1_retic_NYU4-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15592441
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26850.019.81353448
TATCGCG38850.018.73856541
ATGCCGT28200.015.76221549
GTATGCC35400.015.52240647
CGGGGTG19900.014.94946753
CGTATGC31750.014.77170646
TCGTATC197250.014.017338
CGTATCG99700.014.00666739
GTATCGC55900.013.64924740
CGTATCT106000.013.40532139
ATCGCGT29600.013.24316342
CGCGTAT19800.013.08072944
TGCCGGG17050.012.72715550
TCGCGTA22000.012.40901643
ATCGCGG28000.012.37492542
ATGCCGG21300.012.3237949
ATCGGGG23650.012.13523342
TGCCGTC28100.012.08173950
GTATCGG51800.011.82421540
GCGGGGT23150.011.49017452
Produced by FastQC (version 0.11.5)