FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmLG1_retic_NYU3-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmLG1_retic_NYU3-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216523
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22950.010.06511648
CGTATGC27700.08.97097646
CAGAAAT178150.08.13336637
TTCAGAA227850.07.97215870
GTATGCC31850.07.91189647
TCAGAAA195750.07.759678436
CATTCAG262600.07.703545670
AATCGCG21150.07.446658641
AATCTCG45500.07.38446741
ACATTCA320000.07.07639170
ATTCAGA263250.07.019773570
TCACATT392650.06.74757670
GTCACAT404700.06.73692670
CACATTC339050.06.709762669
TCGTATG34600.06.676165645
AGAAATC118650.06.57798538
AGTCACA454950.06.45439970
CAGTCAC480700.06.29795470
CTCGTAT31750.06.28333844
GAAATCT82950.06.075777539
Produced by FastQC (version 0.11.5)