FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmKN1_retic_NYU1-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmKN1_retic_NYU1-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564964
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT178450.1865663059474139TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41800.035.92079548
ATGCCGT42200.032.67749449
TGCCGTC40100.032.55587850
GTATGCC48200.032.09520747
CGTATGC48700.031.40651346
GCCGTCT34450.030.98671751
TCGTATG61600.026.647445
CTCGTAT60150.025.13684744
CCGTCTT49700.022.18294152
TCTCGTA76750.019.79128843
TATCTCG96100.018.428341
ATCTCGT94450.017.9722542
GCGTGAA22450.015.58984561
CGTCTTC63450.015.11407753
ATGCCGG12500.014.83989549
TGCCGGC10700.014.39242250
CACCGCG17750.013.40807470
TTATCTC132750.013.34056240
CTGCTTG46750.012.95157759
CTGCGTG15800.012.84780959
Produced by FastQC (version 0.11.5)