FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmHB54_retic_NYU7-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmHB54_retic_NYU7-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11823038
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT126660.10712982568439684TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42250.034.70999548
ATGCCGT43450.032.78489349
GCCGTCT35950.031.34930651
TGCCGTC44000.030.70475250
CGTATGC50400.029.51421246
GTATGCC53250.027.9999547
TCGTATG63150.023.61011145
CCGTCTT50400.022.56959552
CTCGTAT55150.021.64066744
TCTCGTA66650.017.11903243
GATCTCG77200.014.82485341
CGTCTTC71800.014.38019853
CTGCTTG57450.014.07290359
ATCTCGT79900.014.0173242
GCTTGAA78050.012.01791661
CTTCTGC70550.011.80710856
TGCTTGA85100.09.99410660
TCTGCTT89550.09.65380858
CGGCTAT212150.09.37039470
CTGCGTG14150.09.15181259
Produced by FastQC (version 0.11.5)