FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmHB117_FBR1_retic_NYU5-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmHB117_FBR1_retic_NYU5-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16152171
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT174910.10828884860121898TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58000.031.9217848
CGTATGC62800.029.5384546
ATGCCGT57700.028.93356749
TGCCGTC56900.026.51089750
GTATGCC71200.026.44610447
GCCGTCT48050.025.34809151
TCGTATG78950.024.20508845
CTCGTAT77600.022.77696244
CCGTCTT67750.018.1324852
TCTCGTA94650.017.82348343
TATCTCG109900.016.94254741
ATCTCGT115150.015.80540142
GCTATCT132100.014.67770839
AGCTATC167800.012.55613138
CTATCTC153900.012.39431940
CGTCTTC92150.011.20433453
TGCCGGC16250.010.76901650
ATGCCGG20750.010.62629449
CTGCGTG21650.09.53779859
AAGCTAT265000.09.53548237
Produced by FastQC (version 0.11.5)