FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmBZIP2_DFL1_retic_NYU8-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmBZIP2_DFL1_retic_NYU8-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16302460
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG34500.017.24587442
ATGGGGG62000.014.7334442
AATCGGG54400.013.51063441
AATGGGG81950.012.85502441
GCGAAAT253150.011.682384537
TGCGGGG22300.011.61426151
GCGGGGT26200.011.48846152
CGCGAAA286750.010.6796936
TGGGGGG50650.010.36494943
CGGGGTG25750.010.32998653
CCGCGAA348800.09.90366770
AAATCGG85600.09.85368540
CGAAATG162700.09.65854138
CGAAATC148600.09.46803838
CGGGGGT20300.09.13766544
TCCGCGA406950.09.06473170
CCAGTCC51800.08.91865870
AAATGGG131150.08.85982540
CTCCGCG471300.08.81472370
GAGCACC77200.08.7950870
Produced by FastQC (version 0.11.5)