FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_ZmBBM_retic_NYU2-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_ZmBBM_retic_NYU2-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8939969
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28300.015.21209646
TATGCCG28750.014.85192248
GTATGCC32450.013.80562147
TCGTATG32100.012.75686545
ATGCCGT31650.012.71700449
CTCGTAT29900.012.524940544
TCTCGTA42050.08.82272943
TGCCGTC41400.08.70763150
TTCCATC51550.08.48657238
GATTCCA67550.08.18620736
AGATTCC70350.08.15888335
ATTCCAT60450.08.04765837
ATCTCGT48750.07.6101742
GCCGTCT38800.07.396823451
GAGATTC89200.06.709377334
TCACGAG121150.06.41332169
CGAGATT91900.06.398007433
GCTTGAA35300.06.345572561
CACGAGA121650.06.300650670
CATCTCG56500.06.25653141
Produced by FastQC (version 0.11.5)