FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtTGA5_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtTGA5_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423603
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.018.52875148
GTATGCC4650.016.5585547
TATCGCG5050.015.94035441
TGCCGGG2101.5835223E-514.99946650
GAGCACC11100.014.50398770
ATCGCGT4001.5097612E-1013.99978942
GTATCGC7050.013.90049940
ATGCCGT4051.8553692E-1013.82666849
GGGTGTA3405.0606104E-813.38215261
TGCCGGC2153.239129E-413.02279150
TCGCGTA3002.4406381E-612.8331443
TCGTATC29750.012.823071538
CGTATCT16650.012.61216339
CGTATCG14100.012.41090539
TCGTATG9650.012.33116670
CGTATGC4306.6411303E-912.20911946
CGCGTAT2651.4063953E-411.88661444
GTATCGG7250.011.58603340
GCGGGGT2751.9854083E-411.45413749
CGCGGAT2350.00925841510.42537544
Produced by FastQC (version 0.11.5)