FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtTGA5_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtTGA5_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542744
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG36480.10297103036516328TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG13250.025.35794844
ATCGGGG16950.024.15877542
TCGGGGG16950.023.9522943
AATCGGG22350.018.16516141
CGGGGGT5500.016.54510144
GAATCGG29300.014.69251840
AGAATCG37850.012.02070939
TAGGGGG4501.6311242E-710.88865743
TCCGGAG85300.010.093286570
GGAGAAT60050.09.61650937
GAGAATC49550.09.53534938
CGGAGAA66050.09.32580636
CTCCGGA97000.09.02017169
ACTCCGG105250.08.64565270
CCGGAGA77000.08.63593670
TCGGGGT4502.1383613E-48.55537343
CGGGGTG4000.0060992947.87483244
GAGCACC15600.07.852174370
CACTCCG118050.07.826803770
ATAGGGG8101.260596E-77.77761142
Produced by FastQC (version 0.11.5)