FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtSPL9_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtSPL9_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3663935
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT43050.11749662589538297TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12100.037.02355248
GCCGTCT10200.034.655751
ATGCCGT12000.034.41551249
TGCCGTC11250.034.22107750
GTATGCC14900.029.83121547
CGTATGC14950.029.02991946
CTCGTAT19400.024.35551644
TCGTATG20400.022.4753545
CCGTCTT16650.020.81011452
TCTCGTA25750.019.16492843
TATCTCG29900.018.37781141
ATCTCGT32450.017.25721242
CACCGCG5150.016.31013370
TGCCGGC3151.8579158E-814.4439650
ATGCCGG3707.057679E-1014.18871449
CGTCTTC21200.013.86745853
TTATCTC42350.012.89248140
CTGCGTG4153.921741E-912.65017959
CATTATC50100.012.36485538
ATTATCT49950.011.63124239
Produced by FastQC (version 0.11.5)