FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtSPL9_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtSPL9_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154150
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG6650.024.73589542
CATCGGG10100.018.71215441
GCATCGG12200.017.21245640
CGGGGGT3108.8402885E-1015.80584644
CGCATCG18050.014.93017739
GCGCATG7100.014.78816670
GCGCATC20600.014.78098938
TCGCGCC5700.014.73627970
TCGGGGG5350.013.73779243
CGGGGTG3152.902707E-713.333034553
CGCGCAG6900.013.187901570
CGCGCAT28150.012.68157537
GGCGCTA1950.00230247312.563621517
CATCGCG5451.0913936E-1112.20136741
ATCGCGG4053.7865902E-812.09830342
GGCGGGG5451.6971171E-910.91735951
TCGGGGT5451.6989361E-910.91701343
TCGCGCA38200.010.35300736
CGGGGAT3501.6456771E-49.99961844
CGCAGCG4956.0443926E-79.89861168
Produced by FastQC (version 0.11.5)