FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtSPL11_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtSPL11_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917811
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG51410.13122123553178036TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG19750.024.80945843
ATCGGGG21050.024.44114542
CGGGGGG17500.022.5993944
CGGGGGT5850.020.34133144
AATCGGG28400.017.49952741
GAATCGG36100.013.86389140
TAGGGGG6800.013.38199243
AGAATCG47200.011.12243939
GAGAATC59800.09.77385938
GGAGAAT75150.09.45404837
CGGAGAA87500.08.95987370
ATAGGGG9555.456968E-128.79557442
CCAGTCC10400.08.74987670
TCCGGAG109200.08.62167270
ACTCCGG136300.08.44815570
ATGGGGG9202.3101165E-108.3693442
GAGCACC17850.08.23517770
GGGGGTG17150.08.16325345
TCACTCC181900.08.15821370
CCGGAGA101250.08.15790970
Produced by FastQC (version 0.11.5)