FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtSPL11_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtSPL11_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786220
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG7350.019.52362342
ATGGGGG12850.016.34225542
TGCGGGG4202.1827873E-1114.16615751
AATGGGG17550.013.76055241
AATCGGG12750.012.90183641
GTGGGGG7750.012.64470657
CGGGGGT4458.221832E-1012.58414844
CGGGGTG3952.6733687E-812.40461753
TGGGGGG9850.012.08110243
GCGGGGT4409.3514245E-911.93138852
GCGAAAT52400.011.55507937
GAGCACC15800.011.51872470
CGGGGGG4601.8049832E-811.41263253
TCGGGGG5854.0017767E-1111.36741243
CCGCGAA73250.010.941730570
CGCGAAA59350.010.73269570
TCGGGGT6957.2759576E-1210.57543843
GCGGGGG4401.4354464E-610.34053652
GGTGGGG9750.010.050920556
TGGGGGT8750.09.99990443
Produced by FastQC (version 0.11.5)