FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtLHY_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtLHY_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4038055
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT54260.1343716219813747TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT13900.035.75575651
TGCCGTC15700.035.22328650
TATGCCG16200.034.13614748
ATGCCGT16500.033.7276149
GTATGCC17400.031.37962747
CGTATGC17700.030.65002846
CCGTCTT18900.025.37094152
TCGTATG22950.023.18048945
CTCGTAT20350.022.53010244
CGTCTTC23550.019.6177953
CTGCTTG18900.018.33306559
TCTCGTA26100.017.43247843
GATCTCG26100.016.22561541
CTTCTGC24300.015.987617556
GCTTGAA27150.015.59849161
ATCTCGT30750.013.77198442
TGCTTGA27850.013.44700660
CTGCGTG4203.2923708E-1013.33313859
TGGGGGG4255.5697456E-912.3552081
TTCTGCT30000.011.66663957
Produced by FastQC (version 0.11.5)