FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtLHY_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtLHY_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087278
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG9100.021.5375142
CATCGGG13200.020.15062541
GCATCGG15100.018.77400440
ATGCGCC5100.016.4698670
GCGGGGT3850.016.36397452
GCGCATG8150.016.31829670
CGGGGTG3501.8189894E-1116.0003353
GCGCATC26000.015.34547538
CGCATCG21850.014.73619239
TCGGGGT5450.014.12781643
CGGGGGT4701.0913936E-1113.40366344
GGCGGGG4801.4551915E-1113.12527151
CGGGGTT5307.2759576E-1212.54661644
TGCGCAT37000.012.107573537
CGGGGAT4308.700226E-811.39484644
TCGGGGG6603.6379788E-1211.13587243
TGCGGGG2852.7644658E-411.05285951
GGGGGTG6754.9112714E-1110.3700845
TGGCGGG6805.820766E-1110.2943350
GCGCAGG3401.2279478E-410.29366370
Produced by FastQC (version 0.11.5)