FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtERF1_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtERF1_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478000
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT63300.1413577489950871TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18950.037.123848
ATGCCGT18550.036.22620849
TGCCGTC17300.033.17950
GTATGCC21750.032.827447
CGTATGC23150.030.99334346
GCCGTCT16750.030.50728851
TCGTATG26700.026.21678545
CTCGTAT23700.024.36667844
CCGTCTT23450.022.68643852
TCTCGTA30000.017.26637543
CGCGTAT4550.016.15357244
GATCTCG34200.015.86230541
ATCTCGT33750.015.24418742
CGTCTTC30800.015.11304353
CGATCTC38900.014.21569840
TGCCGGC4051.8553692E-1013.82708250
CTGCTTG22350.013.62393259
CTGCGTG5900.013.05062759
GCGTGAA10000.012.94992661
CTTCTGC28200.012.53496956
Produced by FastQC (version 0.11.5)