FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtERF1_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtERF1_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875307
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT56980.14703351244172397TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15500.037.70919450
TATGCCG17250.036.5212848
ATGCCGT17600.035.19841449
GCCGTCT15000.033.59957551
CGTATGC20500.032.61018446
GTATGCC19950.032.28029347
TCGTATG25450.027.09234245
CCGTCTT20500.023.73140552
CTCGTAT21000.023.66667244
CTGCTTG20100.018.2833559
TCTCGTA25750.017.94175143
CGTCTTC25500.016.88213753
GATCTCG28200.016.13454641
ATCTCGT29650.015.10961542
GCTTGAA29800.014.9161161
TGTCGTA9600.013.48958743
TGCTTGA29250.013.04273760
GTCGTAT10500.012.6666744
CTTCTGC28300.012.36717456
CTGCGTG4501.3038516E-811.66651859
Produced by FastQC (version 0.11.5)