FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtCAMTA1_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtCAMTA1_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924200
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT55750.14206717292696602TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17350.037.92460348
TGCCGTC17150.035.71384450
ATGCCGT17850.034.70545649
GTATGCC19900.033.7684347
CGTATGC20800.032.4755646
GCCGTCT14450.030.51865451
TCGTATG24650.027.8292745
CTCGTAT22350.025.99520544
CCGTCTT20550.021.80021952
TCTCGTA27000.019.96271543
CATCTCG32800.018.13955741
ATCTCGT35800.016.7176742
GCCGTCG9800.016.0712351
CTGCTTG19200.015.13021359
GCGTGAA8750.014.79962761
CCGTCGT10050.014.62668552
CGTCTTC25950.014.0269853
CCATCTC47750.013.04662740
CTGCGTG4651.200533E-1012.79570459
TCCATCT50750.012.13747139
Produced by FastQC (version 0.11.5)