FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtCAMTA1_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtCAMTA1_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755640
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT57110.15206462813262187TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17600.036.98744248
ATGCCGT18550.035.2818849
TGCCGTC18750.033.59891550
GCCGTCT16900.032.9279351
GTATGCC20100.032.38701647
CGTATGC20650.031.86549646
TCGTATG24800.026.10941745
CCGTCTT21850.025.94881652
CTCGTAT24000.024.06300444
CGTCTTC27700.018.19435953
TCTCGTA31450.017.69512443
CTGCTTG21500.017.58082659
GATCTCG33100.016.07284441
CTTCTGC26550.015.4232356
ATCTCGT34300.015.40848542
GCTTGAA31200.014.02291661
TGCTTGA31400.012.70744160
AGATCTC42750.012.60845140
TTCTGCT32000.012.24976757
TCTGCTT34650.011.81795758
Produced by FastQC (version 0.11.5)