FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtARF2_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtARF2_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948220
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG17050.021.96459441
ATCGGGG12800.021.8753442
GCATCGG20450.019.51081340
CGGGGGT5700.018.4213444
GCGCATC35450.016.38870438
CGCATCG30750.016.16203539
TCGGGGT8550.015.55579843
TCGCGCC8600.015.46457370
TCGGGGG8150.015.46036443
CGCGCAT49450.014.0844437
CGGGGTT8000.014.00021844
CGGGGTG5150.013.59227253
TCGCGCA64950.012.12428636
GCGGCGT3003.4381006E-511.666752
GCGCATG11250.011.51070870
CCAGTCC10050.011.143887570
CACCCGG2250.006707247310.88850667
CTCGCGC85650.010.29736170
GCGGGGT5251.2564851E-710.00002952
TCTCGCG101550.09.54668670
Produced by FastQC (version 0.11.5)