FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n01_AtARF2_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n01_AtARF2_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612862
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT47050.13022916457921724TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11900.033.8233148
ATGCCGT12300.030.73150849
GTATGCC14000.030.499847
GCCGTCT9150.029.45336251
CGTATGC14750.028.47439246
TGCCGTC12300.028.1705550
TCGTATG20650.021.0162345
CTCGTAT18550.020.56533444
CCGTCTT15700.016.71963552
TCTCGTA24550.014.68381543
TATCTCG27950.014.40022341
ATCTCGT29900.012.64170842
GCGTGAA6800.011.83783161
GCTATCT33750.011.51071839
CGTCTTC22200.011.50893453
CTGCGTG4105.7828765E-711.0971859
CTGCTTG17850.010.58787359
CTATCTC38850.010.45009240
AGCTATC42250.010.18900138
CCGGCGT3501.6452999E-49.99993452
Produced by FastQC (version 0.11.5)