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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-04-05, 01:57 based on data in: /scratch/gencore/logs/html/H3VCHDRX3/merged


        General Statistics

        Showing 348/348 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H3VCHDRX3_n01_A01ATH
        71.6%
        40%
        4.2
        H3VCHDRX3_n01_A01CHI
        67.7%
        39%
        5.0
        H3VCHDRX3_n01_A02ATH
        79.7%
        40%
        9.6
        H3VCHDRX3_n01_A02CHI
        69.0%
        38%
        5.2
        H3VCHDRX3_n01_A03ATH
        78.8%
        39%
        8.0
        H3VCHDRX3_n01_A03CHI
        60.1%
        39%
        2.9
        H3VCHDRX3_n01_A04ATH
        5.2%
        39%
        0.0
        H3VCHDRX3_n01_A04CHI
        64.8%
        38%
        3.9
        H3VCHDRX3_n01_A05ATH
        67.2%
        39%
        3.2
        H3VCHDRX3_n01_A05CHI
        64.7%
        39%
        3.7
        H3VCHDRX3_n01_A06ATH
        81.5%
        39%
        8.5
        H3VCHDRX3_n01_A06CHI
        72.8%
        39%
        5.3
        H3VCHDRX3_n01_A07ATH
        80.9%
        40%
        8.5
        H3VCHDRX3_n01_A07CHI
        75.6%
        39%
        9.9
        H3VCHDRX3_n01_A08ATH
        74.4%
        39%
        6.0
        H3VCHDRX3_n01_A08CHI
        62.7%
        39%
        2.2
        H3VCHDRX3_n01_A09ATH
        1.4%
        39%
        0.0
        H3VCHDRX3_n01_A09CHI
        78.6%
        39%
        9.5
        H3VCHDRX3_n01_A10ATH
        79.2%
        40%
        7.3
        H3VCHDRX3_n01_A10CHI
        75.6%
        38%
        8.4
        H3VCHDRX3_n01_A11ATH
        82.0%
        39%
        10.1
        H3VCHDRX3_n01_A11CHI
        67.4%
        39%
        4.8
        H3VCHDRX3_n01_A12CHI
        63.0%
        39%
        3.6
        H3VCHDRX3_n01_B01ATH
        67.6%
        40%
        3.2
        H3VCHDRX3_n01_B01CHI
        68.9%
        39%
        5.3
        H3VCHDRX3_n01_B02ATH
        77.3%
        39%
        7.3
        H3VCHDRX3_n01_B02CHI
        64.0%
        40%
        3.6
        H3VCHDRX3_n01_B03ATH
        77.0%
        39%
        6.5
        H3VCHDRX3_n01_B03CHI
        65.3%
        39%
        3.8
        H3VCHDRX3_n01_B04ATH
        8.9%
        40%
        0.0
        H3VCHDRX3_n01_B04CHI
        72.8%
        39%
        5.8
        H3VCHDRX3_n01_B05ATH
        68.9%
        40%
        3.1
        H3VCHDRX3_n01_B05CHI
        68.8%
        39%
        5.2
        H3VCHDRX3_n01_B06ATH
        76.0%
        39%
        4.8
        H3VCHDRX3_n01_B06CHI
        79.8%
        39%
        10.0
        H3VCHDRX3_n01_B07ATH
        79.9%
        40%
        7.2
        H3VCHDRX3_n01_B07CHI
        67.7%
        39%
        4.5
        H3VCHDRX3_n01_B08ATH
        69.9%
        40%
        4.0
        H3VCHDRX3_n01_B08CHI
        79.5%
        39%
        9.8
        H3VCHDRX3_n01_B09ATH
        1.3%
        39%
        0.0
        H3VCHDRX3_n01_B09CHI
        78.5%
        39%
        11.8
        H3VCHDRX3_n01_B10ATH
        81.5%
        39%
        8.3
        H3VCHDRX3_n01_B10CHI
        76.3%
        38%
        9.3
        H3VCHDRX3_n01_B11ATH
        79.5%
        39%
        7.6
        H3VCHDRX3_n01_B11CHI
        74.5%
        39%
        8.7
        H3VCHDRX3_n01_B12CHI
        71.3%
        39%
        6.5
        H3VCHDRX3_n01_C01ATH
        71.3%
        40%
        4.3
        H3VCHDRX3_n01_C01CHI
        63.5%
        39%
        3.9
        H3VCHDRX3_n01_C02ATH
        80.6%
        40%
        9.8
        H3VCHDRX3_n01_C02CHI
        68.9%
        39%
        5.5
        H3VCHDRX3_n01_C03ATH
        76.7%
        39%
        5.8
        H3VCHDRX3_n01_C03CHI
        67.5%
        39%
        4.8
        H3VCHDRX3_n01_C04ATH
        9.0%
        39%
        0.0
        H3VCHDRX3_n01_C04CHI
        70.6%
        39%
        5.7
        H3VCHDRX3_n01_C05ATH
        73.2%
        40%
        4.1
        H3VCHDRX3_n01_C05CHI
        70.4%
        39%
        6.1
        H3VCHDRX3_n01_C06ATH
        74.9%
        40%
        5.1
        H3VCHDRX3_n01_C06CHI
        81.5%
        39%
        14.8
        H3VCHDRX3_n01_C07ATH
        74.5%
        39%
        4.8
        H3VCHDRX3_n01_C07CHI
        75.2%
        39%
        7.9
        H3VCHDRX3_n01_C08ATH
        75.9%
        40%
        5.6
        H3VCHDRX3_n01_C08CHI
        74.2%
        40%
        6.0
        H3VCHDRX3_n01_C09ATH
        0.9%
        39%
        0.0
        H3VCHDRX3_n01_C09CHI
        78.9%
        39%
        11.5
        H3VCHDRX3_n01_C10ATH
        67.8%
        39%
        6.1
        H3VCHDRX3_n01_C10CHI
        73.3%
        39%
        7.9
        H3VCHDRX3_n01_C11ATH
        83.7%
        39%
        12.8
        H3VCHDRX3_n01_C11CHI
        75.9%
        39%
        9.0
        H3VCHDRX3_n01_C12CHI
        68.3%
        39%
        5.0
        H3VCHDRX3_n01_D01ATH
        54.1%
        39%
        1.7
        H3VCHDRX3_n01_D01CHI
        68.7%
        39%
        5.0
        H3VCHDRX3_n01_D02ATH
        77.3%
        39%
        8.3
        H3VCHDRX3_n01_D02CHI
        68.3%
        38%
        5.1
        H3VCHDRX3_n01_D03ATH
        74.9%
        39%
        4.9
        H3VCHDRX3_n01_D03CHI
        68.0%
        39%
        4.4
        H3VCHDRX3_n01_D04ATH
        7.9%
        39%
        0.0
        H3VCHDRX3_n01_D04CHI
        73.5%
        39%
        6.3
        H3VCHDRX3_n01_D05ATH
        71.4%
        40%
        3.7
        H3VCHDRX3_n01_D05CHI
        69.1%
        39%
        5.4
        H3VCHDRX3_n01_D06ATH
        78.5%
        40%
        6.0
        H3VCHDRX3_n01_D06CHI
        76.9%
        39%
        8.0
        H3VCHDRX3_n01_D07ATH
        81.4%
        40%
        8.6
        H3VCHDRX3_n01_D07CHI
        74.5%
        39%
        7.0
        H3VCHDRX3_n01_D08ATH
        83.6%
        39%
        12.9
        H3VCHDRX3_n01_D08CHI
        77.1%
        39%
        8.5
        H3VCHDRX3_n01_D09ATH
        1.3%
        39%
        0.0
        H3VCHDRX3_n01_D09CHI
        76.4%
        40%
        8.4
        H3VCHDRX3_n01_D10ATH
        82.5%
        40%
        9.5
        H3VCHDRX3_n01_D10CHI
        78.1%
        39%
        9.6
        H3VCHDRX3_n01_D11ATH
        82.5%
        40%
        10.8
        H3VCHDRX3_n01_D11CHI
        81.1%
        39%
        14.6
        H3VCHDRX3_n01_D12CHI
        51.8%
        43%
        2.4
        H3VCHDRX3_n01_E01ATH
        67.4%
        39%
        3.0
        H3VCHDRX3_n01_E01CHI
        53.0%
        39%
        2.3
        H3VCHDRX3_n01_E02ATH
        77.0%
        40%
        6.4
        H3VCHDRX3_n01_E02CHI
        65.6%
        39%
        4.6
        H3VCHDRX3_n01_E03ATH
        77.2%
        39%
        5.5
        H3VCHDRX3_n01_E03CHI
        68.3%
        39%
        4.6
        H3VCHDRX3_n01_E04ATH
        8.8%
        40%
        0.0
        H3VCHDRX3_n01_E04CHI
        54.5%
        39%
        2.4
        H3VCHDRX3_n01_E05ATH
        69.3%
        40%
        3.4
        H3VCHDRX3_n01_E05CHI
        59.8%
        39%
        3.9
        H3VCHDRX3_n01_E06ATH
        84.1%
        40%
        11.1
        H3VCHDRX3_n01_E06CHI
        69.9%
        40%
        5.4
        H3VCHDRX3_n01_E07ATH
        74.0%
        39%
        4.2
        H3VCHDRX3_n01_E07CHI
        70.5%
        39%
        5.9
        H3VCHDRX3_n01_E08ATH
        66.5%
        40%
        2.9
        H3VCHDRX3_n01_E08CHI
        76.0%
        39%
        6.3
        H3VCHDRX3_n01_E09ATH
        1.3%
        39%
        0.0
        H3VCHDRX3_n01_E09CHI
        73.8%
        39%
        6.9
        H3VCHDRX3_n01_E10ATH
        77.1%
        39%
        5.1
        H3VCHDRX3_n01_E10CHI
        66.4%
        39%
        4.3
        H3VCHDRX3_n01_E11ATH
        82.6%
        39%
        9.9
        H3VCHDRX3_n01_E11CHI
        72.4%
        39%
        8.4
        H3VCHDRX3_n01_E12CHI
        66.1%
        39%
        4.4
        H3VCHDRX3_n01_F01ATH
        71.6%
        39%
        4.3
        H3VCHDRX3_n01_F01CHI
        65.2%
        39%
        4.0
        H3VCHDRX3_n01_F02ATH
        66.6%
        39%
        3.0
        H3VCHDRX3_n01_F02CHI
        68.0%
        39%
        5.2
        H3VCHDRX3_n01_F03ATH
        74.4%
        39%
        6.0
        H3VCHDRX3_n01_F03CHI
        52.4%
        39%
        2.5
        H3VCHDRX3_n01_F04ATH
        7.9%
        39%
        0.0
        H3VCHDRX3_n01_F04CHI
        59.5%
        39%
        4.0
        H3VCHDRX3_n01_F05ATH
        72.7%
        39%
        4.3
        H3VCHDRX3_n01_F05CHI
        65.5%
        39%
        4.9
        H3VCHDRX3_n01_F06ATH
        77.6%
        39%
        5.7
        H3VCHDRX3_n01_F06CHI
        68.6%
        39%
        4.6
        H3VCHDRX3_n01_F07ATH
        84.4%
        40%
        12.2
        H3VCHDRX3_n01_F07CHI
        70.9%
        39%
        5.5
        H3VCHDRX3_n01_F08ATH
        78.1%
        40%
        6.8
        H3VCHDRX3_n01_F08CHI
        78.5%
        39%
        7.9
        H3VCHDRX3_n01_F09ATH
        1.4%
        39%
        0.0
        H3VCHDRX3_n01_F09CHI
        48.0%
        44%
        1.5
        H3VCHDRX3_n01_F10ATH
        71.6%
        40%
        3.8
        H3VCHDRX3_n01_F11ATH
        83.9%
        39%
        12.0
        H3VCHDRX3_n01_G01ATH
        76.6%
        40%
        6.5
        H3VCHDRX3_n01_G01CHI
        71.0%
        39%
        6.3
        H3VCHDRX3_n01_G02ATH
        84.3%
        39%
        14.6
        H3VCHDRX3_n01_G02CHI
        65.0%
        40%
        4.5
        H3VCHDRX3_n01_G03ATH
        81.5%
        39%
        9.8
        H3VCHDRX3_n01_G03CHI
        69.7%
        39%
        5.1
        H3VCHDRX3_n01_G04ATH
        8.9%
        39%
        0.0
        H3VCHDRX3_n01_G04CHI
        74.5%
        39%
        6.9
        H3VCHDRX3_n01_G05ATH
        75.0%
        40%
        5.3
        H3VCHDRX3_n01_G05CHI
        71.3%
        39%
        6.4
        H3VCHDRX3_n01_G06ATH
        83.4%
        40%
        10.9
        H3VCHDRX3_n01_G06CHI
        79.0%
        39%
        9.4
        H3VCHDRX3_n01_G07ATH
        85.4%
        40%
        14.1
        H3VCHDRX3_n01_G07CHI
        71.7%
        39%
        6.7
        H3VCHDRX3_n01_G08ATH
        80.5%
        40%
        8.3
        H3VCHDRX3_n01_G08CHI
        79.8%
        39%
        10.3
        H3VCHDRX3_n01_G09ATH
        7.9%
        41%
        0.0
        H3VCHDRX3_n01_G09CHI
        80.5%
        39%
        12.6
        H3VCHDRX3_n01_G10ATH
        86.3%
        40%
        16.1
        H3VCHDRX3_n01_H01ATH
        68.8%
        39%
        3.7
        H3VCHDRX3_n01_H01CHI
        66.2%
        39%
        4.4
        H3VCHDRX3_n01_H02ATH
        82.6%
        39%
        11.0
        H3VCHDRX3_n01_H02CHI
        70.2%
        39%
        5.3
        H3VCHDRX3_n01_H03ATH
        79.5%
        39%
        7.6
        H3VCHDRX3_n01_H03CHI
        65.8%
        39%
        3.9
        H3VCHDRX3_n01_H04ATH
        8.8%
        39%
        0.0
        H3VCHDRX3_n01_H04CHI
        72.8%
        39%
        5.9
        H3VCHDRX3_n01_H05ATH
        74.0%
        40%
        5.0
        H3VCHDRX3_n01_H05CHI
        71.1%
        39%
        6.1
        H3VCHDRX3_n01_H06ATH
        79.5%
        39%
        7.0
        H3VCHDRX3_n01_H06CHI
        74.1%
        39%
        6.1
        H3VCHDRX3_n01_H07ATH
        82.0%
        40%
        9.6
        H3VCHDRX3_n01_H07CHI
        69.9%
        39%
        5.3
        H3VCHDRX3_n01_H08ATH
        81.8%
        40%
        9.1
        H3VCHDRX3_n01_H08CHI
        77.2%
        39%
        6.6
        H3VCHDRX3_n01_H09ATH
        2.8%
        39%
        0.0
        H3VCHDRX3_n01_H09CHI
        81.2%
        39%
        13.0
        H3VCHDRX3_n01_H10ATH
        82.0%
        40%
        9.0
        H3VCHDRX3_n01_undetermined
        80.9%
        40%
        98.3
        H3VCHDRX3_n02_A01ATH
        69.8%
        40%
        4.2
        H3VCHDRX3_n02_A01CHI
        65.7%
        39%
        5.0
        H3VCHDRX3_n02_A02ATH
        78.7%
        40%
        9.6
        H3VCHDRX3_n02_A02CHI
        67.0%
        39%
        5.2
        H3VCHDRX3_n02_A03ATH
        77.4%
        39%
        8.0
        H3VCHDRX3_n02_A03CHI
        59.2%
        39%
        2.9
        H3VCHDRX3_n02_A04ATH
        5.3%
        40%
        0.0
        H3VCHDRX3_n02_A04CHI
        62.7%
        38%
        3.9
        H3VCHDRX3_n02_A05ATH
        66.4%
        39%
        3.2
        H3VCHDRX3_n02_A05CHI
        62.8%
        39%
        3.7
        H3VCHDRX3_n02_A06ATH
        80.4%
        39%
        8.5
        H3VCHDRX3_n02_A06CHI
        69.6%
        39%
        5.3
        H3VCHDRX3_n02_A07ATH
        80.4%
        40%
        8.5
        H3VCHDRX3_n02_A07CHI
        75.2%
        39%
        9.9
        H3VCHDRX3_n02_A08ATH
        73.7%
        39%
        6.0
        H3VCHDRX3_n02_A08CHI
        61.6%
        39%
        2.2
        H3VCHDRX3_n02_A09ATH
        1.7%
        39%
        0.0
        H3VCHDRX3_n02_A09CHI
        76.4%
        39%
        9.5
        H3VCHDRX3_n02_A10ATH
        80.5%
        40%
        7.3
        H3VCHDRX3_n02_A10CHI
        74.5%
        38%
        8.4
        H3VCHDRX3_n02_A11ATH
        81.6%
        40%
        10.1
        H3VCHDRX3_n02_A11CHI
        66.5%
        39%
        4.8
        H3VCHDRX3_n02_A12CHI
        62.0%
        39%
        3.6
        H3VCHDRX3_n02_B01ATH
        65.8%
        40%
        3.2
        H3VCHDRX3_n02_B01CHI
        66.9%
        39%
        5.3
        H3VCHDRX3_n02_B02ATH
        76.7%
        40%
        7.3
        H3VCHDRX3_n02_B02CHI
        62.1%
        40%
        3.6
        H3VCHDRX3_n02_B03ATH
        75.9%
        39%
        6.5
        H3VCHDRX3_n02_B03CHI
        64.1%
        39%
        3.8
        H3VCHDRX3_n02_B04ATH
        9.1%
        39%
        0.0
        H3VCHDRX3_n02_B04CHI
        71.3%
        39%
        5.8
        H3VCHDRX3_n02_B05ATH
        68.8%
        40%
        3.1
        H3VCHDRX3_n02_B05CHI
        67.6%
        39%
        5.2
        H3VCHDRX3_n02_B06ATH
        74.1%
        40%
        4.8
        H3VCHDRX3_n02_B06CHI
        77.7%
        39%
        10.0
        H3VCHDRX3_n02_B07ATH
        78.8%
        40%
        7.2
        H3VCHDRX3_n02_B07CHI
        66.7%
        39%
        4.5
        H3VCHDRX3_n02_B08ATH
        69.2%
        40%
        4.0
        H3VCHDRX3_n02_B08CHI
        78.9%
        39%
        9.8
        H3VCHDRX3_n02_B09ATH
        1.5%
        40%
        0.0
        H3VCHDRX3_n02_B09CHI
        77.8%
        39%
        11.8
        H3VCHDRX3_n02_B10ATH
        81.0%
        39%
        8.3
        H3VCHDRX3_n02_B10CHI
        75.8%
        38%
        9.3
        H3VCHDRX3_n02_B11ATH
        78.7%
        39%
        7.6
        H3VCHDRX3_n02_B11CHI
        74.9%
        39%
        8.7
        H3VCHDRX3_n02_B12CHI
        69.6%
        39%
        6.5
        H3VCHDRX3_n02_C01ATH
        69.3%
        40%
        4.3
        H3VCHDRX3_n02_C01CHI
        60.4%
        39%
        3.9
        H3VCHDRX3_n02_C02ATH
        79.7%
        40%
        9.8
        H3VCHDRX3_n02_C02CHI
        67.8%
        39%
        5.5
        H3VCHDRX3_n02_C03ATH
        75.5%
        40%
        5.8
        H3VCHDRX3_n02_C03CHI
        67.0%
        39%
        4.8
        H3VCHDRX3_n02_C04ATH
        8.8%
        39%
        0.0
        H3VCHDRX3_n02_C04CHI
        70.2%
        39%
        5.7
        H3VCHDRX3_n02_C05ATH
        71.5%
        40%
        4.1
        H3VCHDRX3_n02_C05CHI
        69.8%
        39%
        6.1
        H3VCHDRX3_n02_C06ATH
        72.8%
        40%
        5.1
        H3VCHDRX3_n02_C06CHI
        80.5%
        39%
        14.8
        H3VCHDRX3_n02_C07ATH
        71.8%
        40%
        4.8
        H3VCHDRX3_n02_C07CHI
        73.5%
        39%
        7.9
        H3VCHDRX3_n02_C08ATH
        74.6%
        40%
        5.6
        H3VCHDRX3_n02_C08CHI
        72.7%
        40%
        6.0
        H3VCHDRX3_n02_C09ATH
        1.1%
        39%
        0.0
        H3VCHDRX3_n02_C09CHI
        77.1%
        39%
        11.5
        H3VCHDRX3_n02_C10ATH
        67.4%
        39%
        6.1
        H3VCHDRX3_n02_C10CHI
        72.6%
        39%
        7.9
        H3VCHDRX3_n02_C11ATH
        82.3%
        40%
        12.8
        H3VCHDRX3_n02_C11CHI
        74.2%
        39%
        9.0
        H3VCHDRX3_n02_C12CHI
        65.2%
        39%
        5.0
        H3VCHDRX3_n02_D01ATH
        58.6%
        39%
        1.7
        H3VCHDRX3_n02_D01CHI
        64.5%
        39%
        5.0
        H3VCHDRX3_n02_D02ATH
        76.1%
        40%
        8.3
        H3VCHDRX3_n02_D02CHI
        66.1%
        39%
        5.1
        H3VCHDRX3_n02_D03ATH
        74.6%
        40%
        4.9
        H3VCHDRX3_n02_D03CHI
        66.1%
        39%
        4.4
        H3VCHDRX3_n02_D04ATH
        7.9%
        39%
        0.0
        H3VCHDRX3_n02_D04CHI
        71.3%
        39%
        6.3
        H3VCHDRX3_n02_D05ATH
        70.6%
        40%
        3.7
        H3VCHDRX3_n02_D05CHI
        66.0%
        39%
        5.4
        H3VCHDRX3_n02_D06ATH
        76.8%
        40%
        6.0
        H3VCHDRX3_n02_D06CHI
        73.7%
        39%
        8.0
        H3VCHDRX3_n02_D07ATH
        80.9%
        40%
        8.6
        H3VCHDRX3_n02_D07CHI
        72.5%
        39%
        7.0
        H3VCHDRX3_n02_D08ATH
        81.6%
        39%
        12.9
        H3VCHDRX3_n02_D08CHI
        75.1%
        40%
        8.5
        H3VCHDRX3_n02_D09ATH
        1.2%
        40%
        0.0
        H3VCHDRX3_n02_D09CHI
        73.6%
        40%
        8.4
        H3VCHDRX3_n02_D10ATH
        82.1%
        40%
        9.5
        H3VCHDRX3_n02_D10CHI
        76.4%
        39%
        9.6
        H3VCHDRX3_n02_D11ATH
        81.6%
        40%
        10.8
        H3VCHDRX3_n02_D11CHI
        78.9%
        39%
        14.6
        H3VCHDRX3_n02_D12CHI
        46.2%
        44%
        2.4
        H3VCHDRX3_n02_E01ATH
        65.0%
        40%
        3.0
        H3VCHDRX3_n02_E01CHI
        52.6%
        39%
        2.3
        H3VCHDRX3_n02_E02ATH
        76.3%
        40%
        6.4
        H3VCHDRX3_n02_E02CHI
        65.7%
        39%
        4.6
        H3VCHDRX3_n02_E03ATH
        76.8%
        39%
        5.5
        H3VCHDRX3_n02_E03CHI
        66.9%
        39%
        4.6
        H3VCHDRX3_n02_E04ATH
        8.6%
        39%
        0.0
        H3VCHDRX3_n02_E04CHI
        58.1%
        39%
        2.4
        H3VCHDRX3_n02_E05ATH
        68.8%
        40%
        3.4
        H3VCHDRX3_n02_E05CHI
        62.1%
        39%
        3.9
        H3VCHDRX3_n02_E06ATH
        83.0%
        40%
        11.1
        H3VCHDRX3_n02_E06CHI
        71.7%
        40%
        5.4
        H3VCHDRX3_n02_E07ATH
        72.2%
        40%
        4.2
        H3VCHDRX3_n02_E07CHI
        70.4%
        39%
        5.9
        H3VCHDRX3_n02_E08ATH
        65.2%
        40%
        2.9
        H3VCHDRX3_n02_E08CHI
        74.5%
        39%
        6.3
        H3VCHDRX3_n02_E09ATH
        1.4%
        39%
        0.0
        H3VCHDRX3_n02_E09CHI
        71.6%
        39%
        6.9
        H3VCHDRX3_n02_E10ATH
        76.7%
        40%
        5.1
        H3VCHDRX3_n02_E10CHI
        63.8%
        39%
        4.3
        H3VCHDRX3_n02_E11ATH
        81.6%
        40%
        9.9
        H3VCHDRX3_n02_E11CHI
        74.6%
        39%
        8.4
        H3VCHDRX3_n02_E12CHI
        62.8%
        39%
        4.4
        H3VCHDRX3_n02_F01ATH
        70.0%
        39%
        4.3
        H3VCHDRX3_n02_F01CHI
        63.6%
        39%
        4.0
        H3VCHDRX3_n02_F02ATH
        63.9%
        40%
        3.0
        H3VCHDRX3_n02_F02CHI
        67.5%
        39%
        5.2
        H3VCHDRX3_n02_F03ATH
        76.8%
        39%
        6.0
        H3VCHDRX3_n02_F03CHI
        57.0%
        39%
        2.5
        H3VCHDRX3_n02_F04ATH
        8.3%
        39%
        0.0
        H3VCHDRX3_n02_F04CHI
        67.5%
        39%
        4.0
        H3VCHDRX3_n02_F05ATH
        70.9%
        40%
        4.3
        H3VCHDRX3_n02_F05CHI
        66.9%
        39%
        4.9
        H3VCHDRX3_n02_F06ATH
        76.9%
        40%
        5.7
        H3VCHDRX3_n02_F06CHI
        68.0%
        39%
        4.6
        H3VCHDRX3_n02_F07ATH
        83.7%
        40%
        12.2
        H3VCHDRX3_n02_F07CHI
        69.5%
        39%
        5.5
        H3VCHDRX3_n02_F08ATH
        77.5%
        40%
        6.8
        H3VCHDRX3_n02_F08CHI
        76.6%
        39%
        7.9
        H3VCHDRX3_n02_F09ATH
        1.7%
        39%
        0.0
        H3VCHDRX3_n02_F09CHI
        46.7%
        44%
        1.5
        H3VCHDRX3_n02_F10ATH
        73.3%
        40%
        3.8
        H3VCHDRX3_n02_F11ATH
        82.9%
        39%
        12.0
        H3VCHDRX3_n02_G01ATH
        75.2%
        40%
        6.5
        H3VCHDRX3_n02_G01CHI
        68.9%
        39%
        6.3
        H3VCHDRX3_n02_G02ATH
        83.3%
        40%
        14.6
        H3VCHDRX3_n02_G02CHI
        62.4%
        40%
        4.5
        H3VCHDRX3_n02_G03ATH
        80.9%
        39%
        9.8
        H3VCHDRX3_n02_G03CHI
        67.8%
        39%
        5.1
        H3VCHDRX3_n02_G04ATH
        8.8%
        39%
        0.0
        H3VCHDRX3_n02_G04CHI
        71.9%
        39%
        6.9
        H3VCHDRX3_n02_G05ATH
        74.1%
        40%
        5.3
        H3VCHDRX3_n02_G05CHI
        70.5%
        39%
        6.4
        H3VCHDRX3_n02_G06ATH
        82.0%
        40%
        10.9
        H3VCHDRX3_n02_G06CHI
        76.9%
        39%
        9.4
        H3VCHDRX3_n02_G07ATH
        84.9%
        40%
        14.1
        H3VCHDRX3_n02_G07CHI
        69.7%
        39%
        6.7
        H3VCHDRX3_n02_G08ATH
        79.6%
        40%
        8.3
        H3VCHDRX3_n02_G08CHI
        78.1%
        39%
        10.3
        H3VCHDRX3_n02_G09ATH
        7.7%
        41%
        0.0
        H3VCHDRX3_n02_G09CHI
        78.5%
        39%
        12.6
        H3VCHDRX3_n02_G10ATH
        85.9%
        40%
        16.1
        H3VCHDRX3_n02_H01ATH
        67.3%
        40%
        3.7
        H3VCHDRX3_n02_H01CHI
        66.2%
        39%
        4.4
        H3VCHDRX3_n02_H02ATH
        81.9%
        40%
        11.0
        H3VCHDRX3_n02_H02CHI
        68.7%
        39%
        5.3
        H3VCHDRX3_n02_H03ATH
        78.6%
        39%
        7.6
        H3VCHDRX3_n02_H03CHI
        64.3%
        39%
        3.9
        H3VCHDRX3_n02_H04ATH
        8.7%
        39%
        0.0
        H3VCHDRX3_n02_H04CHI
        70.9%
        39%
        5.9
        H3VCHDRX3_n02_H05ATH
        73.7%
        40%
        5.0
        H3VCHDRX3_n02_H05CHI
        70.2%
        39%
        6.1
        H3VCHDRX3_n02_H06ATH
        77.9%
        40%
        7.0
        H3VCHDRX3_n02_H06CHI
        71.1%
        39%
        6.1
        H3VCHDRX3_n02_H07ATH
        81.6%
        40%
        9.6
        H3VCHDRX3_n02_H07CHI
        67.9%
        39%
        5.3
        H3VCHDRX3_n02_H08ATH
        80.4%
        40%
        9.1
        H3VCHDRX3_n02_H08CHI
        75.1%
        39%
        6.6
        H3VCHDRX3_n02_H09ATH
        3.0%
        40%
        0.0
        H3VCHDRX3_n02_H09CHI
        79.9%
        39%
        13.0
        H3VCHDRX3_n02_H10ATH
        81.7%
        40%
        9.0
        H3VCHDRX3_n02_undetermined
        78.0%
        41%
        98.3

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 174/174 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        98257858
        8.6
        A01ATH
        4211858
        0.4
        B01ATH
        3238678
        0.3
        C01ATH
        4289782
        0.4
        D01ATH
        1671670
        0.1
        E01ATH
        3025815
        0.3
        F01ATH
        4284644
        0.4
        G01ATH
        6453562
        0.6
        H01ATH
        3713643
        0.3
        A02ATH
        9618820
        0.8
        B02ATH
        7266697
        0.6
        C02ATH
        9801562
        0.9
        D02ATH
        8349913
        0.7
        E02ATH
        6385113
        0.6
        F02ATH
        3013181
        0.3
        G02ATH
        14615623
        1.3
        H02ATH
        11015046
        1.0
        A03ATH
        8038427
        0.7
        B03ATH
        6457274
        0.6
        C03ATH
        5807925
        0.5
        D03ATH
        4936898
        0.4
        E03ATH
        5499676
        0.5
        F03ATH
        5956598
        0.5
        G03ATH
        9799370
        0.9
        H03ATH
        7599404
        0.7
        A04ATH
        3933
        0.0
        B04ATH
        8657
        0.0
        C04ATH
        7792
        0.0
        D04ATH
        7822
        0.0
        E04ATH
        7343
        0.0
        F04ATH
        8313
        0.0
        G04ATH
        11671
        0.0
        H04ATH
        10495
        0.0
        A05ATH
        3226952
        0.3
        B05ATH
        3091451
        0.3
        C05ATH
        4116132
        0.4
        D05ATH
        3711473
        0.3
        E05ATH
        3388404
        0.3
        F05ATH
        4317025
        0.4
        G05ATH
        5294127
        0.5
        H05ATH
        5008446
        0.4
        A06ATH
        8524699
        0.7
        B06ATH
        4773009
        0.4
        C06ATH
        5107308
        0.4
        D06ATH
        6036667
        0.5
        E06ATH
        11126610
        1.0
        F06ATH
        5666943
        0.5
        G06ATH
        10867429
        0.9
        H06ATH
        6998477
        0.6
        A07ATH
        8484664
        0.7
        B07ATH
        7165027
        0.6
        C07ATH
        4784104
        0.4
        D07ATH
        8645084
        0.8
        E07ATH
        4166044
        0.4
        F07ATH
        12177855
        1.1
        G07ATH
        14101394
        1.2
        H07ATH
        9634334
        0.8
        A08ATH
        5965059
        0.5
        B08ATH
        3955415
        0.3
        C08ATH
        5608914
        0.5
        D08ATH
        12865660
        1.1
        E08ATH
        2881086
        0.3
        F08ATH
        6792586
        0.6
        G08ATH
        8318954
        0.7
        H08ATH
        9118148
        0.8
        A09ATH
        9571
        0.0
        B09ATH
        6694
        0.0
        C09ATH
        6538
        0.0
        D09ATH
        6887
        0.0
        E09ATH
        5422
        0.0
        F09ATH
        7394
        0.0
        G09ATH
        22620
        0.0
        H09ATH
        8375
        0.0
        A10ATH
        7343723
        0.6
        B10ATH
        8283486
        0.7
        C10ATH
        6057936
        0.5
        D10ATH
        9544376
        0.8
        E10ATH
        5094144
        0.4
        F10ATH
        3807819
        0.3
        G10ATH
        16063109
        1.4
        H10ATH
        8992837
        0.8
        A11ATH
        10076816
        0.9
        B11ATH
        7585298
        0.7
        C11ATH
        12782485
        1.1
        D11ATH
        10808475
        0.9
        E11ATH
        9945391
        0.9
        F11ATH
        12036800
        1.1
        A01CHI
        4958899
        0.4
        B01CHI
        5316138
        0.5
        C01CHI
        3895740
        0.3
        D01CHI
        4992737
        0.4
        E01CHI
        2281048
        0.2
        F01CHI
        3989109
        0.3
        G01CHI
        6340041
        0.6
        H01CHI
        4352393
        0.4
        A02CHI
        5203306
        0.5
        B02CHI
        3619987
        0.3
        C02CHI
        5517749
        0.5
        D02CHI
        5118962
        0.4
        E02CHI
        4621611
        0.4
        F02CHI
        5218280
        0.5
        G02CHI
        4453107
        0.4
        H02CHI
        5302021
        0.5
        A03CHI
        2899327
        0.3
        B03CHI
        3801152
        0.3
        C03CHI
        4788583
        0.4
        D03CHI
        4356833
        0.4
        E03CHI
        4606946
        0.4
        F03CHI
        2494255
        0.2
        G03CHI
        5052152
        0.4
        H03CHI
        3877436
        0.3
        A04CHI
        3908416
        0.3
        B04CHI
        5834231
        0.5
        C04CHI
        5685561
        0.5
        D04CHI
        6293347
        0.6
        E04CHI
        2394591
        0.2
        F04CHI
        3967426
        0.3
        G04CHI
        6882086
        0.6
        H04CHI
        5866666
        0.5
        A05CHI
        3737091
        0.3
        B05CHI
        5216290
        0.5
        C05CHI
        6146556
        0.5
        D05CHI
        5385136
        0.5
        E05CHI
        3872052
        0.3
        F05CHI
        4870721
        0.4
        G05CHI
        6367971
        0.6
        H05CHI
        6099489
        0.5
        A06CHI
        5256478
        0.5
        B06CHI
        9995264
        0.9
        C06CHI
        14815637
        1.3
        D06CHI
        8041262
        0.7
        E06CHI
        5375028
        0.5
        F06CHI
        4593132
        0.4
        G06CHI
        9404303
        0.8
        H06CHI
        6094693
        0.5
        A07CHI
        9923053
        0.9
        B07CHI
        4541549
        0.4
        C07CHI
        7946391
        0.7
        D07CHI
        7015309
        0.6
        E07CHI
        5908202
        0.5
        F07CHI
        5507912
        0.5
        G07CHI
        6737806
        0.6
        H07CHI
        5287501
        0.5
        A08CHI
        2189822
        0.2
        B08CHI
        9834776
        0.9
        C08CHI
        6005507
        0.5
        D08CHI
        8534047
        0.7
        E08CHI
        6263224
        0.5
        F08CHI
        7865623
        0.7
        G08CHI
        10348825
        0.9
        H08CHI
        6606710
        0.6
        A09CHI
        9491799
        0.8
        B09CHI
        11812563
        1.0
        C09CHI
        11495151
        1.0
        D09CHI
        8377891
        0.7
        E09CHI
        6879523
        0.6
        F09CHI
        1495552
        0.1
        G09CHI
        12618686
        1.1
        H09CHI
        13035563
        1.1
        A10CHI
        8394401
        0.7
        B10CHI
        9294136
        0.8
        C10CHI
        7926616
        0.7
        D10CHI
        9556064
        0.8
        E10CHI
        4289072
        0.4
        A11CHI
        4791996
        0.4
        B11CHI
        8727983
        0.8
        C11CHI
        9027489
        0.8
        D11CHI
        14641459
        1.3
        E11CHI
        8407956
        0.7
        A12CHI
        3558987
        0.3
        B12CHI
        6476757
        0.6
        C12CHI
        4976402
        0.4
        D12CHI
        2358463
        0.2
        E12CHI
        4418361
        0.4

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here. If your libraries are dual indexed, the two indicies are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        27764869.0
        28.3
        GAGCTCGAGTGTATAT
        4895802.0
        5.0
        CGTCATACGGGGGGGG
        1068879.0
        1.1
        CATCGTGAGGGGGGGG
        875030.0
        0.9
        AGCATACCGGGGGGGG
        795821.0
        0.8
        ATAGCGCTGGGGGGGG
        770762.0
        0.8
        ATGAGCTCGGGGGGGG
        607446.0
        0.6
        CTCTAGAGGGGGGGGG
        541657.0
        0.6
        CAGTAGGTGGGGGGGG
        513012.0
        0.5
        TCAGTCTAGGGGGGGG
        503694.0
        0.5
        CGAGCTAGGGGGGGGG
        498822.0
        0.5
        TCTAGACTGGGGGGGG
        465099.0
        0.5
        GAGCTCGAGGGGGGGG
        456525.0
        0.5
        GGGGGGGGCGAGACGT
        439655.0
        0.5
        TCCTCATGGGGGGGGG
        432309.0
        0.4
        GGGGGGGGCAGATAGT
        400453.0
        0.4
        GGGGGGGGTAGTAACG
        368851.0
        0.4
        GGGGGGGGACGGTGTC
        355341.0
        0.4
        GGGGGGGGCGTACGAT
        327747.0
        0.3
        GGGGGGGGACACGATC
        311567.0
        0.3

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        2.0
        1276674048
        1143521105
        8.6
        2.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (151bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        348 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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