FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14323551
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT6966984.864003346656147No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC5979934.17489350231657No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT5091193.554418872806052No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA4943523.4513229296282746No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG4103992.8652043058317034No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT3981692.779820450948232No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG3857712.693263702555323No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA3530352.4647170244306036No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT3338462.330748848522269No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA3320042.317888908972363No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC2690891.878647271196926No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC2594671.8114711917456783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2264691.5810953582669551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG749850.0142.183231
GATCTCT759950.0141.906227
CACGATC752300.0141.670884
ACACGAT754000.0141.64723
CGCATCT564200.0141.526288
ACGATCT754700.0141.344255
GCATCTT565250.0141.314279
ATCGCGC366200.0141.30152
CGATTCC393250.0141.281373
TCGTTAC654500.0141.255785
TCGATTC394600.0141.193632
TACACGA755650.0141.113562
GATTCCT394600.0140.943864
ATGGTCT447450.0140.773936
ATTCCTT396150.0140.646595
ACTGTAT560800.0140.564073
TCGCATC565400.0140.538997
TATCTCT581050.0140.529027
AATGGTC448850.0140.447025
AGAGTCT417550.0140.380636
Produced by FastQC (version 0.11.5)