FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_plasmid.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_plasmid.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC5816.95906432748538No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT4513.157894736842104No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT339.649122807017543No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA267.602339181286549No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA257.309941520467836No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG195.555555555555555No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT195.555555555555555No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG174.970760233918129No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA154.385964912280701No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC133.8011695906432745No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT72.046783625730994No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT72.046783625730994No Hit
GATAGCTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA61.7543859649122806No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC61.7543859649122806No Hit
ATACTCGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT20.5847953216374269No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG10.29239766081871343No Hit
AATATAGAGAGTTAAGGTCTTGTGGAAAGGACGAAACACCGACAGTATTT10.29239766081871343No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTA10.29239766081871343No Hit
GATAGCTCGGATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
GATCGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAAGACCGCCAGTAGGTCC10.29239766081871343No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAACGCCAGTAGGTCCACTATG10.29239766081871343No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAAGTAGGCCACAAAT10.29239766081871343No Hit
CGATCATGATCGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGAACAAT10.29239766081871343No Hit
TAACAATCGTCTAGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG10.29239766081871343No Hit
TAAGTATAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG10.29239766081871343No Hit
GATAGGTCGCATCTTGTGGAAAGTACGAACCCCCGCCAGTAGGTCCACTA10.29239766081871343No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAATATGGGCACATTT10.29239766081871343No Hit
ATAAAGATTAAATTTTGCATGGGACAAAACACCGCCAGGGCCACAAGGTT10.29239766081871343No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCT10.29239766081871343No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCGTAGGTACAC10.29239766081871343No Hit
GATAGGTCGCATCTTGTTGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
CGATCATGAAACTATTGTAAAAAACAATAAACACCCCCAATATCTCCAAT10.29239766081871343No Hit
TAACAATGGTCTTGTGGAAAGGCCGAAACACCGCCCGTAGGTCCACTATG10.29239766081871343No Hit
ATACACGATCTCTTGTGGAAGGGACGAAACACCGCCAGTAGGTCCACTAT10.29239766081871343No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAACT10.29239766081871343No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA10.29239766081871343No Hit
GATAGGTCGCATCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
TACCAATGGTATGTTCTAAAGGAATAAACACATCAAGTAGATAAACTAGC10.29239766081871343No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCGTTGGTG10.29239766081871343No Hit
CGATCGATACAGGAATTCTTGTGGAAAGAAACAAACACAGCCAGTACGAA10.29239766081871343No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGCCGTTGGTGCACG10.29239766081871343No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT10.29239766081871343No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCCC10.29239766081871343No Hit
AGGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT10.29239766081871343No Hit
ATACTGTATCTCTTGTGGAAAGGTCGAAACACCGCCAGTAGGTCCACTAT10.29239766081871343No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCTAGTAGGTCCACTAT10.29239766081871343No Hit
GATAGCGCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACAGCCAGTAGGTCCACTA10.29239766081871343No Hit
CGATCGATACAGGTAATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC10.29239766081871343No Hit
AATCTATAACGCAAATCTATTGTAAAAAACGAAACACCCCCACAATATCA10.29239766081871343No Hit
TAAGAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG10.29239766081871343No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTA10.29239766081871343No Hit
GATAGCTCGCATCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
ATAAAATAAAACTATTGTAAAAAACAAAACACACCCACAATATCAACAAT10.29239766081871343No Hit
ATACAAGATCTCTTGTGGAAAGGACGAAACACCGCCGTAGGTCCACTATG10.29239766081871343No Hit
GATCGCGCAGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.29239766081871343No Hit
ATACTCTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT10.29239766081871343No Hit
GTTAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAATA10.29239766081871343No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAC200.0045089128.890-94
ACTAGTT200.0045089128.895-99
TTGAAAA200.0045089128.8100-104
AAAGTGG200.0045089128.8105-109
AGCTGTG200.0045089128.880-84
TGTAAGC200.0045089128.885-89
Produced by FastQC (version 0.11.5)