FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_plasmid_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_plasmid_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8874429
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT139239815.690001013022922No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG105206611.855027517826782No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC97929611.035031098902252No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG8263319.311370906229573No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA7224428.140715306866504No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA6402127.214120480314847No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6106786.881321603902628No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT5786396.520295559297392No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT5526596.227544329894352No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC4387424.943889911114281No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC4315134.862431149091395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG1477800.0143.160521
CACGATC1480100.0142.768774
GATCTCT1483850.0142.709037
AAGTAGA1110750.0142.675572
CGATCTC1483200.0142.654886
AGAGTCT1112850.0142.559986
ACACGAT1485250.0142.5213
TAGAGTC1113300.0142.45065
ACGATCT1485850.0142.385915
TACACGA1486400.0142.38172
ATCTCTT1492950.0142.364848
ATCGCGC682950.0142.252622
TCTCTTG1493450.0142.235219
CGCGCGG683250.0142.22664
AACAATG885500.0142.194272
CGATCGT1048850.0142.135962
CGTTACC1047700.0142.118076
TTACCAT1052300.0142.070248
AGGTCGC779550.0142.052024
GATCGCG684500.0142.026441