Basic Statistics
Measure | Value |
---|---|
Filename | H3V73AFX2_n01_plasmid_corrected.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8874429 |
Sequences flagged as poor quality | 0 |
Sequence length | 150 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT | 1392398 | 15.690001013022922 | No Hit |
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG | 1052066 | 11.855027517826782 | No Hit |
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC | 979296 | 11.035031098902252 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG | 826331 | 9.311370906229573 | No Hit |
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA | 722442 | 8.140715306866504 | No Hit |
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA | 640212 | 7.214120480314847 | No Hit |
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA | 610678 | 6.881321603902628 | No Hit |
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT | 578639 | 6.520295559297392 | No Hit |
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT | 552659 | 6.227544329894352 | No Hit |
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC | 438742 | 4.943889911114281 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC | 431513 | 4.862431149091395 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATACACG | 147780 | 0.0 | 143.16052 | 1 |
CACGATC | 148010 | 0.0 | 142.76877 | 4 |
GATCTCT | 148385 | 0.0 | 142.70903 | 7 |
AAGTAGA | 111075 | 0.0 | 142.67557 | 2 |
CGATCTC | 148320 | 0.0 | 142.65488 | 6 |
AGAGTCT | 111285 | 0.0 | 142.55998 | 6 |
ACACGAT | 148525 | 0.0 | 142.521 | 3 |
TAGAGTC | 111330 | 0.0 | 142.4506 | 5 |
ACGATCT | 148585 | 0.0 | 142.38591 | 5 |
TACACGA | 148640 | 0.0 | 142.3817 | 2 |
ATCTCTT | 149295 | 0.0 | 142.36484 | 8 |
ATCGCGC | 68295 | 0.0 | 142.25262 | 2 |
TCTCTTG | 149345 | 0.0 | 142.23521 | 9 |
CGCGCGG | 68325 | 0.0 | 142.2266 | 4 |
AACAATG | 88550 | 0.0 | 142.19427 | 2 |
CGATCGT | 104885 | 0.0 | 142.13596 | 2 |
CGTTACC | 104770 | 0.0 | 142.11807 | 6 |
TTACCAT | 105230 | 0.0 | 142.07024 | 8 |
AGGTCGC | 77955 | 0.0 | 142.05202 | 4 |
GATCGCG | 68450 | 0.0 | 142.02644 | 1 |