FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8981
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT102511.412982964035185No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC7558.406636232045429No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA6647.3933860371896225No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT6507.23750139182719No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT6016.69190513305868No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG5856.513751252644472No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG5746.391270459859704No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA4915.467097205211001No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT4324.81015477118361No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA3924.364770070148091No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC3774.197750807259771No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC2763.0731544371450843No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.757153991760383No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT160.17815388041420777No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA100.11134617525887987No Hit
GATAGCTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA90.10021155773299187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATC600.0144.775285
AACAATG700.0144.775282
CAATGGT700.0144.775284
CGATAGG359.094947E-12144.775286
CGTTACC800.0144.775286
TCGATAC450.0144.775284
TCGCGCG400.0144.775283
ATGGTCT700.0144.775286
GCGCGGT400.0144.775285
AATGGTC700.0144.775285
TCATGAT600.0144.775284
TAACAAT700.0144.775281
TCGTTAC800.0144.775285
GATCATG600.0144.775282
GATTCCT400.0144.775284
CGCGGTT400.0144.775286
ATCATGA600.0144.775283
ATCGTTA800.0144.775284
ATTCCTT400.0144.775285
CGCGCGG400.0144.775284
Produced by FastQC (version 0.11.5)