FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_prestim_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_prestim_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9963397
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT111500411.19100242618055No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC107846710.824290149233239No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT9850089.886266702009365No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA8550888.582293769885913No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT7809617.838300531435212No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG7493767.521290178440145No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6882616.907894967951192No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6734086.758819306306875No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT6482646.50645557935712No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA5929835.951614695269093No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC5353165.372826155577259No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC4549734.566444557012031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT1054100.0143.195311
ATCTCTT2244450.0142.668988
ATACACG1176800.0142.592771
AGGTCGC937800.0142.472094
GATCTCT1181750.0142.379627
TCTCTTG2246950.0142.375669
CGATCTC1179600.0142.30626
CACGATC1178400.0142.267794
CGTTACC1168750.0142.265696
GCGCGGT629050.0142.183915
CGCATCT945600.0142.172848
CGCGCGG628300.0142.147344
ACTGTAT1063050.0141.990113
ATTCCTT733300.0141.923845
CGCGGTT632950.0141.910786
TACCATC1175000.0141.910729
ATCGATT735350.0141.904391
CGATTCC731450.0141.898863
TACACGA1183400.0141.88362
TTACCAT1175950.0141.875678
Produced by FastQC (version 0.11.5)