FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85840
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT996011.602982292637465No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC896210.4403541472507No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT872410.16309412861137No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA76248.881640260950606No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG64187.476700838769804No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT63347.378844361602982No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG58556.8208294501397955No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA57566.705498602050326No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA53006.174277726001864No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT47455.5277260018639325No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC43305.0442684063373715No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC42414.940587138863001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT6050.0144.000026
GCGCGGT6100.0144.05
CATGATC6600.0144.05
ATACTGT10700.0144.01
TGAATCT100.00722765144.05
ATCTCTT22400.0143.357158
TACTGTA10800.0143.333332
ACTGTAT10800.0143.333333
GTTACCA8600.0143.162787
GCATCTT7850.0143.08289
AGGTCGC7850.0143.08284
ATAGGTC7750.0143.070972
TCTCTTG22450.0143.037869
AATGGTC6850.0142.94895
ACAATGG6850.0142.94893
CGATTCC6750.0142.933333
TCATGAT6650.0142.91734
CGCGCGG6150.0142.829274
CGGTTCT6100.0142.819678
ACACGAT11300.0142.725663
Produced by FastQC (version 0.11.5)