FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_presort_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_presort_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803767
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT114672011.696728410620121No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC102648810.470342675422621No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT98119010.008295790791438No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA8635308.808144869212008No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT7361687.509031987398314No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG7334907.481715956733774No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6631816.764552849940232No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6549746.680840130125492No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT6394356.52233983120978No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA5969606.089088000561417No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC5106655.208865122967529No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC4989435.089298837885478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT1047350.0143.170521
ATACACG1205150.0142.736821
ATCTCTT2265450.0142.528148
GATCTCT1208750.0142.51057
CGATCTC1207450.0142.484446
ACTGTAT1053150.0142.464143
CGCGCGG651050.0142.463554
TCTCTTG2265250.0142.372279
CACGATC1206700.0142.322364
AGGTCGC943650.0142.321384
GCGCGGT653000.0142.247655
CGCATCT950650.0142.227398
CGTTACC1105500.0142.212276
TAGAGTC713050.0142.003315
GATCGCG655250.0141.991071
ACGATCT1211950.0141.937565
ACACGAT1212600.0141.932743
CTGTATC1055500.0141.928634
AGAGTCT713300.0141.923266
AAGTAGA712950.0141.91792
Produced by FastQC (version 0.11.5)