FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_CFSE_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_CFSE_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC24411.423220973782772No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT23511.00187265917603No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT23010.767790262172285No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA1868.707865168539326No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT1647.677902621722846No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA1507.02247191011236No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG1456.788389513108614No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG1446.741573033707865No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA1316.132958801498127No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC1225.711610486891386No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC1215.664794007490636No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT1185.52434456928839No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT30.1404494382022472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAT252.674824E-8144.000023
AACGCAT100.006736223144.000028
CGATAAC100.006736223144.000024
CGTTACC252.674824E-8144.000026
TCGATCG201.7280836E-6144.000021
TCGATAA100.006736223144.000023
TTACCAT252.674824E-8144.000028
ACGCATT100.006736223144.000029
GTCGCAT400.0144.000026
ATAGGTC400.0144.000022
TAACGCA100.006736223144.000027
GTATCTC252.674824E-8144.000026
GATCGTT252.674824E-8144.000023
GATAACG100.006736223144.000025
ATAACGC100.006736223144.000026
TATCTCT252.674824E-8144.000027
TACTGTA252.674824E-8144.000022
TCGCATC400.0144.000027
TCGTTAC252.674824E-8144.000025
AGGTCGC400.0144.000024
Produced by FastQC (version 0.11.5)