FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_CFSE_low_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_CFSE_low_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8873044
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT104768411.80749244565901No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC96268610.849557378505054No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT91069510.263614155412732No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA7889648.891694890727466No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6826267.693256113685449No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT6293507.092830825588152No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6195246.982090926180463No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6113966.890487638740436No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT5736206.464748737862677No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA5445046.136608812037898No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC4495955.066975887868921No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC3594454.050977319621091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT973900.0143.595231
CGCGCGG583450.0143.433034
ATACACG1097800.0143.096681
AACAATG726150.0143.073682
GCGCGGT585400.0143.016745
CGATCTC1097700.0143.015176
GATCTCT1099700.0143.007547
ATCTCTT2085900.0142.842938
CGCATCT859350.0142.821358
ATCGCGC587300.0142.814162
TCTCTTG2083700.0142.800239
CACGATC1099100.0142.76754
ACACGAT1101250.0142.724173
TAACAAT727250.0142.694371
CGTTACC1031850.0142.615696
GCATCTT862100.0142.566229
AGGTCGC857200.0142.50494
GGTCGCA857250.0142.471395
TACACGA1103350.0142.41682
ACAATGG727350.0142.401753
Produced by FastQC (version 0.11.5)