FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_CFSE_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_CFSE_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41153
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT529212.859329817996256No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA414710.07702962116978No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT39769.661507059023645No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC36688.91308045585984No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG27446.66780064636843No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT25806.269287779748743No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA21475.217116613612617No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG21325.180667266055938No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA20444.966831093723422No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT17964.364201880786334No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC13183.2026826719801713No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC13163.197822758972614No Hit
GATAGCTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA1630.3960829101159089No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT1310.3183243019949943No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA960.23327582436274394No Hit
ATACTCTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT720.17495686827205792No Hit
ATACTGGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT680.1652370422569436No Hit
AAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG520.1263577381964863No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT440.10691808616625763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTC5500.0144.16792
GATAACG1700.0144.16795
TCTATCT100.007188956144.16795
TTCCTTG1600.0144.16796
ATAGCTC202.1673332E-6144.16792
CTCGCAT202.1673332E-6144.16796
ACAATGC100.007188956144.16793
ATGGTCT2450.0144.167886
TACTGGA151.2498355E-4144.167882
TGGATCT151.2498355E-4144.167885
AGGTCGC5450.0144.167884
ATACTGT5050.0144.167881
ACTGGAT151.2498355E-4144.167883
CTGTATC5100.0144.167884
CGATCTC6100.0144.167886
GGTCGCA5450.0144.167885
GCTCTTG100.0072151655143.992728
ATACAGG1150.0143.992727
ACGCATT1750.0143.99279
TAACGCA1750.0143.99277
Produced by FastQC (version 0.11.5)