FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD8_CFSE_high_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD8_CFSE_high_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608928
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT111478011.60150226955598No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC102864910.705137971686332No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT97620710.159374698197343No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA8404518.746563612507035No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG7297547.594541243310387No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT7157627.44892666486834No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG6615166.884389184724872No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA6543676.809989626314195No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA5838326.075932715907539No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT5461935.684224088264581No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC4960885.162781946123438No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC4955455.157130951548393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT1052200.0143.407491
AGGTCGC915850.0143.343264
ATCTCTT2232900.0143.02128
CACGATC1171750.0143.017754
CGCGCGG623850.0142.784364
ACGATCT1174450.0142.774785
TCTCTTG2234550.0142.751289
CGTTACC1103300.0142.746736
GCGCGGT625000.0142.740555
CGGTTCT629250.0142.697048
CGATCTC1176000.0142.66626
GATCTCT1177200.0142.637547
CGCATCT926900.0142.595498
TACTGTA1058150.0142.590472
GATTCCT706250.0142.520634
ATACACG1178800.0142.517321
ACTGTAT1058550.0142.475253
ATCGCGC626400.0142.449362
ATTCCTT707500.0142.401155
GCGGTTC628350.0142.397257
Produced by FastQC (version 0.11.5)