FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT4510.368663594470046No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA4410.138248847926267No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC4410.138248847926267No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT4410.138248847926267No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA429.67741935483871No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT347.834101382488479No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG337.603686635944701No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG296.682027649769585No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC245.529953917050691No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT245.529953917050691No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC225.0691244239631335No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA204.6082949308755765No Hit
TGACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG20.4608294930875576No Hit
ATTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGA10.2304147465437788No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCCGCCAGTAGGTCCACTA10.2304147465437788No Hit
CGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT10.2304147465437788No Hit
ATCCACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT10.2304147465437788No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT10.2304147465437788No Hit
TAATAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG10.2304147465437788No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCTC10.2304147465437788No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGATCCACTA10.2304147465437788No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTTGA10.2304147465437788No Hit
CGATCGAAACTGGTACTCTTGTCAAGACCGCAAAACCCCCCCACGAGCAC10.2304147465437788No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGATCCACT10.2304147465437788No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.2304147465437788No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAAACACCGCCAGTAGGT10.2304147465437788No Hit
GGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT10.2304147465437788No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGAGGTC10.2304147465437788No Hit
TAAGTGGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG10.2304147465437788No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCATAGGTCCACTATG10.2304147465437788No Hit
GATCGCTCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.2304147465437788No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAAAACCGCCAGTAGGTCCACTATG10.2304147465437788No Hit
TAAAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGA10.2304147465437788No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCTACTATG10.2304147465437788No Hit
TCTATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC10.2304147465437788No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGTCCACTATG10.2304147465437788No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGAAACACCGCCAGTAGGTCCA10.2304147465437788No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCGTAGGTCCACT10.2304147465437788No Hit
TAACAATGGTCTTGTGGAAAGGGCGAAACACCGCCAGTAGGTCCACTATG10.2304147465437788No Hit
ATACACGATCTCTTGTGGAAAGGACTAAACACCGCCAGTAGGTCCACTAT10.2304147465437788No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGG155.2308314E-5144.07
TCGATTC155.2308314E-5144.02
CGATACA100.004769927144.05
TACAGGT100.004769927144.08
CGTTACC100.004769927144.06
TCGATAC100.004769927144.04
TTACCAT100.004769927144.08
GATACAG100.004769927144.06
GATCGTT100.004769927144.03
CCTTGGT155.2308314E-5144.08
CTTGGTT155.2308314E-5144.09
TTCCTTG155.2308314E-5144.06
TCGTTAC100.004769927144.05
GATTCCT155.2308314E-5144.04
ATCGTTA100.004769927144.04
ATTCCTT155.2308314E-5144.05
ACAGGTA100.004769927144.09
TACCATC100.004769927144.09
ATCGATT155.2308314E-5144.01
ATCGATA100.004769927144.03
Produced by FastQC (version 0.11.5)