FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_prestim_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_prestim_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11606258
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT132057011.378085856785193No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC123244110.618762739894288No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT11490829.900538140716844No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10227788.81229764149651No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT8781587.566245727089645No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG8688747.486254398273759No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG7838896.75402011569965No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA7817126.735262993464388No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT7675066.612863508634738No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA7108566.124764760528329No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC6123475.276007133393037No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC5910835.092795628013783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT1230750.0142.958971
ATCTCTT2666650.0142.66448
ATACACG1423750.0142.597171
ACTGTAT1234950.0142.529863
GATCTCT1425800.0142.475867
AGGTCGC1103200.0142.44954
GATCGCG763050.0142.388431
CGATCTC1424350.0142.384576
CGCGCGG759700.0142.363834
TCTCTTG2669950.0142.315489
CGCATCT1110800.0142.296198
TCGCATC1110250.0142.152667
CGTTACC1311700.0142.123116
ACACGAT1429050.0142.117843
TAGAGTC835550.0142.040395
CGATTCC859450.0142.028473
GATCATG949600.0141.967792
ATCGATT864800.0141.943661
ATCGCGC764450.0141.933472
AACAATG926550.0141.909092
Produced by FastQC (version 0.11.5)