FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA1010.204081632653061No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC99.183673469387756No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG99.183673469387756No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG88.16326530612245No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT88.16326530612245No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT88.16326530612245No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC77.142857142857142No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT77.142857142857142No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC66.122448979591836No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA66.122448979591836No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA55.1020408163265305No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT44.081632653061225No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA11.0204081632653061No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATGA11.0204081632653061No Hit
GATAGGTCGCATCTTCTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA11.0204081632653061No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT11.0204081632653061No Hit
ATAGAATGATCATCTAGTAAAGAAGAAAAAACACCAAAAGGAGCAACAAT11.0204081632653061No Hit
CGATCTTGATCCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT11.0204081632653061No Hit
ATCTATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA11.0204081632653061No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCAAGTAGGTCAAC11.0204081632653061No Hit
TATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC11.0204081632653061No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGTACACAAAT11.0204081632653061No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACCCCGCCAGTAGGTCCACT11.0204081632653061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA50.0144.05
TACAGGT50.0144.08
TCGATAC50.0144.04
GATACAG50.0144.06
GATCGAT50.0144.02
ACAGGTA50.0144.09
ATCGATA50.0144.03
ATACAGG50.0144.07
CGATCGA50.0144.01
Produced by FastQC (version 0.11.5)