Basic Statistics
Measure | Value |
---|---|
Filename | H3V73AFX2_n01_CD4_presort_corrected.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11485284 |
Sequences flagged as poor quality | 0 |
Sequence length | 150 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT | 1338242 | 11.651797204144016 | No Hit |
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC | 1229667 | 10.706457062794442 | No Hit |
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT | 1153132 | 10.0400825961291 | No Hit |
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA | 1016918 | 8.854095379792088 | No Hit |
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG | 866031 | 7.5403533774175715 | No Hit |
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT | 822869 | 7.164550741627286 | No Hit |
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG | 788272 | 6.863321795090134 | No Hit |
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA | 775114 | 6.748757801722621 | No Hit |
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT | 752646 | 6.553133557689997 | No Hit |
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA | 696561 | 6.06481302508497 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC | 585423 | 5.097157371119425 | No Hit |
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC | 555141 | 4.8334982400086925 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATACTGT | 123160 | 0.0 | 142.97284 | 1 |
CGCGCGG | 74715 | 0.0 | 142.53307 | 4 |
ATCTCTT | 268180 | 0.0 | 142.53302 | 8 |
ATACACG | 143530 | 0.0 | 142.48244 | 1 |
ACTGTAT | 123625 | 0.0 | 142.47372 | 3 |
GATCTCT | 143895 | 0.0 | 142.35538 | 7 |
AGGTCGC | 109050 | 0.0 | 142.34665 | 4 |
TCTCTTG | 268270 | 0.0 | 142.31612 | 9 |
CGATCTC | 143835 | 0.0 | 142.19997 | 6 |
CGCATCT | 109825 | 0.0 | 142.13316 | 8 |
TAGAGTC | 84815 | 0.0 | 142.11551 | 5 |
AGAGTCT | 84810 | 0.0 | 142.08992 | 6 |
CGTTACC | 132365 | 0.0 | 142.05882 | 6 |
CTGTATC | 123785 | 0.0 | 142.05106 | 4 |
TCGATTC | 83765 | 0.0 | 142.04187 | 2 |
CACGATC | 143730 | 0.0 | 142.0233 | 4 |
ACACGAT | 144035 | 0.0 | 142.01752 | 3 |
ACGATCT | 143930 | 0.0 | 142.01106 | 5 |
AACAATG | 93000 | 0.0 | 141.92764 | 2 |
TACTGTA | 124145 | 0.0 | 141.91655 | 2 |