FastQCFastQC Report
Thu 12 Mar 2020
H3V73AFX2_n01_CD4_presort_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3V73AFX2_n01_CD4_presort_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485284
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT133824211.651797204144016No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC122966710.706457062794442No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT115313210.0400825961291No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10169188.854095379792088No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG8660317.5403533774175715No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT8228697.164550741627286No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG7882726.863321795090134No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA7751146.748757801722621No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT7526466.553133557689997No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA6965616.06481302508497No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC5854235.097157371119425No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC5551414.8334982400086925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT1231600.0142.972841
CGCGCGG747150.0142.533074
ATCTCTT2681800.0142.533028
ATACACG1435300.0142.482441
ACTGTAT1236250.0142.473723
GATCTCT1438950.0142.355387
AGGTCGC1090500.0142.346654
TCTCTTG2682700.0142.316129
CGATCTC1438350.0142.199976
CGCATCT1098250.0142.133168
TAGAGTC848150.0142.115515
AGAGTCT848100.0142.089926
CGTTACC1323650.0142.058826
CTGTATC1237850.0142.051064
TCGATTC837650.0142.041872
CACGATC1437300.0142.02334
ACACGAT1440350.0142.017523
ACGATCT1439300.0142.011065
AACAATG930000.0141.927642
TACTGTA1241450.0141.916552